It is stable in a broad range of environments: pH, buffer salts, detergents (SDS), and temperature. 1010, Hubtown Solaris, NS Phadke marg, Andheri (E), Mumbai 400069. Please note that this is different to a website ordering account. harvested rnas were quantified using a nanodrop microspectrophotometer, and cdna was prepared using the high capacity cdna transcription kit (thermo h_k@`+H >e>xI Sw;;uR'a+bO :3YfhZ462Hh. International International International hbbd``b`j V`,ACX2$!be `AXW3012,q)'3` ( +44(0) 115 977 5060, There are currently no items in your basket, Scientific Laboratory Supplies Ltd 2022, Proteinase K from Tritirachium album, lyophilized powder, BioUltra, >=30 units/mg protein, for molecular biology. : 39450-01-6. sigma. 443496*6: Glicina ultra pura 500mg Catalogo 13-1323-05 marca LGC biotecnologia: 2,00: UN. This solution is stable for 6 month at 4 C. Procedure: Deparaffinize sections in 2 changes of xylene, 5 minutes each. Proteinase K, Lyophilized MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents. Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad (Asp39-His69-Ser224). MDL Number: MFCD00132129 Sigma P5568, supplied by Millipore, used in various techniques. SDS and urea will denature . 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It is used for the removal of endotoxins bound to cationic proteins such as lysozyme and ribonuclease A. Please fill out the form below if you want to request literature from Krackeler Scientific. If you'd like the most current information sooner, please don't hesitate to drop us an email or give us a call and we'd be happy to assist. Proteinase K Stock Solution (20x) ------ 1 ml TE-CaCl2 Buffer, pH8.0 ------------------ 19 ml Mix well. Proteinase K is a stable and highly reactive serine protease. Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad (Asp39-His69-Ser224). Optical properties of the actual product may deviate. Proteinase K from Tritirachium album. Compare Product No . In the presence of 0.1-0.5% SDS, proteinase K retains activity and will digest a variety of proteins and nucleases in DNA preparations without compromising the integrity of the isolated DNA. Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA. In the presence of 0.1-0.5% SDS, proteinase K retains activity and will digest a variety of proteins and nucleases in DNA preparations without compromising the integrity of the isolated DNA. Suspicious activity from your network has been detected by one of our administrators. It is stable in a broad range of environments: pH, buffer salts, detergents (SDS), and temperature. This pipeline enables the quantitative analyses of chromatin fiber interactions without compromising its sensitivity down to <300 cells, making it suitable for MiSeq analyses of higher-order. buffered . It cleaves peptide bonds adjacent to the carboxylic group of aliphatic and aromatic amino acids and is useful for general digestion of protein in biological . This product is a chemical and requires you to have an SLS credit account. Product Line. P2308, Sigma) was crystalized by mixing a 1:1 ratio of 40 mg ml 1 protein solution in 20 mM MES - NaOH (pH 6.5) and. One unit will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0mole of tyrosine per min at pH7.5 at 37C (color by Folin-Ciocalteu reagent). Sigma-Aldrich offers Roche-RPROTK-RO, Proteinase K, recombinant, PCR Grade lyophilizate from Pichia pastoris for your research needs. Proteinase K from Tritirachium album, lyophilized powder, BioUltra, >=30 units/mg protein, for molecular biology Code: P2308-100MG D2-231 Application Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA.Removes endotoxins that bind to cationic proteins such as lysozyme and ri. Deoxyribonuclease I from bovine pancreas catalogo D5025-150KU marca Sigma: 1,00: UN. Enzyme Commission number: 3.4.21.64 (BRENDA, IUBMB) Numer EC: This image depicts SKU: P2308-1G, This picture is provided solely for illustration purposes. endstream endobj 22 0 obj <> endobj 23 0 obj <>/MediaBox[0 0 612.12 792.12]/Parent 19 0 R/Resources<>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI]/XObject<>>>/Rotate 0/Tabs/S/Type/Page>> endobj 24 0 obj <>stream 2 0 obj One unit will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0mole of tyrosine per min at pH7.5 at 37C (color by Folin-Ciocalteu reagent). Synonym: Endopeptidase K. CAS Number: 39450-01-6 . This product is a chemical and requires you to have an SLS credit account. Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad (Asp39-His69-Ser224). %PDF-1.3 Proteinase K is a stable and highly reactive serine protease. Proteinase K from Engyodontium album (formerly Tritirachium album) for Molecular Biology Catalog Number P2308 Storage Temperature -20 C CAS RN 39450-01-6 E.C. Synonym(s): Endopeptidase K. CAS No. Please include your IP address, which is 45.32.126.163. Determination of enzyme localization on membranes. Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA. It is stable in a broad range of environments: pH, buffer salts, detergents (SDS), and temperature. Proteinase K Inhibitor - Calbiochem Synonym (s): Proteinase K Inhibitor - Calbiochem Empirical Formula (Hill Notation): C26H35ClN4O7 Molecular Weight: 551.03 Match Criteria: Product Name, Keyword All Photos ( 2) Proteinase K-Agarose from Tritirachium album Match Criteria: Product Name, Keyword All Photos ( 1) IHC Select Proteinase K lyophilized powder, BioUltra, 30 units/mg protein, for molecular biology . endstream endobj startxref 443496*7: Ponceau S solution 1 L Catalogo: P7170 marca: Sigma : 1,00: UN. :RbZ1@y. The Muse de Grenoble, right in the heart of the city, has an astonishing collection of 900 works of fine . Applied Filters: Keyword:'p2308' Showing 1-1 of 1 result for " p2308 " within Products. This product is being discontinued, you may only order up to the available stock. However, If you are found to be in breach of the terms and conditions of this website, we reserve the right to take further action. One unit will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0mole of tyrosine per min at pH7.5 at 37C (color by Folin-Ciocalteu reagent). The region now has a handful of airports taking international flights. Search or Browse for items and add to them to your Shopping Cart. Not available outside of the UK & Ireland. Proteinase K is a stable and highly reactive serine protease. P4850. Buy online Proteinase K-100mg (from Tritirachium album) 30 units/mg protein P2308-100MG 39450-01-6 at best price in India on Biomall. stream . Millipore proteinase k Proteinase K, supplied by Millipore, used in various techniques. sigma. Cubic Insulin Removes endotoxins that bind to cationic proteins such as lysozyme and ribonuclease A. Application Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA.Removes endotoxins that bind to cationic proteins such as lysozyme and ri. endobj Proteinase K from Tritirachium album lyophilized powder, BioUltra, 30 units/mg protein, for molecular biology; CAS Number: 39450-01-6; EC Number: 254-457-8; Synonyms: Endopeptidase K; find Sigma-Aldrich-P2308 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich /Length 57 Proteinase K is active in a wide range of temperatures and buffers with optimal activity between 20 and 60C and a pH between 7.5 and 12.0 (1, 2). Collectively, these findings own significant implications for deciphering the biological basis of ADHD. This enzyme belongs to Peptidase family S8 ( subtilisin ). <> +44(0) 115 977 5060, There are currently no items in your basket, Scientific Laboratory Supplies Ltd 2022, Proteinase K from Tritirachium album, lyophilized powder, BioUltra, >=30 units/mg protein, for molecular biology. Please note that this is different to a website ordering account. United States Globally. Proteinase KKDNaseRNaseDNA >30U/mg Product Type: Chemical. Relevant product information is printed on labeled products and other accompanying or available information material. P2308. Proteinase K is able to digest hair ( keratin ), hence, the name "Proteinase K". 4ml. One unit will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0mole of tyrosine per min at pH7.5 at 37C (color by Folin-Ciocalteu reagent). Concentration: Generally proteinase K is used in the concentration range of 50 to 500 g/mL at 65 degrees C in the presence of SDS (0.5-1%). +44(0) 28 9262 1100, Republic of Ireland Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad (Asp. Proteinase K is useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA. % International . 1 0 obj Please contact us to arrange this for you. Proteinase K K CNNEW REVIEWED: 4126.76: -+ . It is stable in a broad range of environments: pH, buffer salts, detergents (SDS), and temperature. It is useful for the isolation of hepatic, yeast, and mung bean mitochondria and is used to determine enzyme localization on membranes #HKO3tXO0ofQ*D'6bsy3N`,aepHqXN&on%v7%RzG/|4 QZBz,n]% 5K@v7G}"#5\MXq`Pq3cDb?-^`f7\S{U%.M-YSPRgQ"4nUL$6\E7sRu2>Ai`j:W2e!=e!d~YhihfAj;7yI8Gv_ED6&[ 8 ls"3vK. We also observed that in mammals Syn III exerts this function acting upstream of brain-derived neurotrophic factor (BDNF)- and cAMP-dependent protein kinase 5 (Cdk5)-stimulated dendrite development. P2308-100MG Synonym: Endopeptidase K CAS Number: 39450-01-6 EC Number: 254-457-8 Enzyme Commission (EC) Number: 3.4.21.64 MDL number: MFCD00132129 Biochem/physiol Actions Proteinase K is a stable and highly reactive serine protease. 4 0 obj read more ADD 77.70 25MG List Price OVERVIEW Not available outside of the UK & Ireland. : 39450-01-6 1.2 Relevant identified uses of the substance or mixture and uses advised against Identified uses : Laboratory chemicals, Manufacture of substances 1.3 Details of the supplier of the safety data sheet All Photos (3) Proteinase . SIGMA-ALDRICH CORPORATION Part No. Sigma recommends storage at -20 C, though others have claimed stability at 2-8 C. 2.3. It is commonly used for its broad specificity. Click "Request Quote" at the bottom of the page. Application Digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research. >> /Length 4989 stream Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA.Removes endotoxins that bind to cationic proteins such as lysozyme and ribonuclease A.Reported useful for the isolation of hepatic, yeast, and mung bean mitochondriaDetermination of enzyme localization on membranesTreatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling.Digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research. . x\vnq R#M8k'vSk-jVI9Oi8"F8ysj?>w#W=+~7wj~~/Rb*bE//_\_xo9f_0SuST5=(G`/~\|F?f}5+ U48hSWp7j_vy)R.%/}?hYNU4,G/_}+O*X?eEsn~oZrCy# Sigma-Aldrich (1) Physical Form. << This product is being discontinued, you may only order up to the available stock. 0 Click the "Request Quote" button at the bottom of the Shopping Cart page. endobj Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad (Asp 39-His 69-Ser 224). Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad (Asp39-His69-Ser224). If you place an order, you will be acknowledged with any corrected pricing. It possesses a high specific activity over a wide range of temperatures and pH values with substantially increased activity at higher temperature. 21 0 obj <> endobj endstream Please note that this is different to a website ordering account. and quantity. Optical properties of the actual product may deviate. $*l}rdeuP5?^O,_f-e/C@@ O\Bi a! P2308. It is particularly suitable for short digestion times. Proteinase K is a stable and highly reactive serine protease. Results Addition ofL-carnitine to the culture medium significantly increased the OCRs of morulae and improved the morphologically-good blastocyst formation rate per zygote compared with sibling embryos. Similar to the growth of Lysozyme control crystals, proteinase K control crystals were grown with no agarose and the addition of nucleating solution was in the liquid state. Please contact us to arrange this for you. Proteinase K from Tritirachium album. Please contact us to arrange this for you. : 647-014-00-9 CAS-No. . This image depicts SKU: P2308-500MG, Krackeler Scientific 2013-2022. Temperature optimum: 65 degrees C; 12X more active at 65 degrees C than at 25 degrees C. pH: Proteinase K is stable over a wide pH range (4.0 to 12.5), with optimal activity at pH 6.5 to 9.5. Copyright CiteAb document.write((new Date()).getFullYear()). Once the solution cools down, filter through a 0.45-m filter and store at 4C until ready for use. Chemical Composition. : 254-457-8. Quality Level. endobj P2308 Proteinase K from Tritirachium album 5.0 lyophilized powder, BioUltra, 30 units/mg protein, for molecular biology Synonym (s): Endopeptidase K CAS Number: 39450-01-6 Enzyme Commission number: 3.4.21.64 ( BRENDA, IUBMB) EC Number: 254-457-8 MDL number: MFCD00132129 eCl@ss: 32160410 NACRES: NA.54 One unit will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0mole of tyrosine per min at pH7.5 at 37C (color by Folin-Ciocalteu reagent). The specifications of the recombinant enzyme are the same as those of the native protease. Add multiple items to your shopping cart by entering the Krackeler Cat.No. stream Please note that this is different to a website ordering account. 3.4.21.64 Synonyms: Peptidase K, Endoproteinase K, Endopeptidase K Temperature prof Product Description Proteinase K is a stable serine protease with broad substrate specificity. This may include detection of an attempt to scrape CiteAb.com, or another breach of our Terms & Conditions of use. Proteinase K from Tritirachium album. Copyrights 2022 Chargen Life Sciences LLP. +44(0) 115 982 1111, Northern Ireland Available for Sale. We are currently working diligently to update our website pricing information for the New Year. Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA.Removes endotoxins that bind to cationic proteins such as lysozyme and ribonuclease A.Reported useful for the isolation of hepatic, yeast, and mung bean mitochondriaDetermination of enzyme localization on membranesTreatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling.Digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research. Arriving at the region's main airport of Lyon . Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA.Removes endotoxins that bind to cationic proteins such as lysozyme and ribonuclease A.Reported useful for the isolation of hepatic, yeast, and mung bean mitochondriaDetermination of enzyme localization on membranesTreatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling.Digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research. Optical properties of the actual product may deviate. Sigma-Aldrich (1) Physical Form. Your price: Log in. Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad (Asp39-His69-Ser224). Use the "Add More Lines" button to add as many lines as you need. Sort by Relevance. This product is being discontinued, you may only order up to the available stock. Proteinase K is a stable and highly reactive serine protease. %PDF-1.5 solution on a stirring hot plate in a fume hood. Proteinase K (PK) Solution. 1 0 obj % Keywords: Code: P2308-25MG D2-231 Not available outside of the UK & Ireland. ZERO BIAS . +44(0) 115 977 5060, There are currently no items in your basket, Scientific Laboratory Supplies Ltd 2022. All Rights Reserved, P261 - P264 - P280 - P284 - P302 + P352 - P305 + P351 + P338. When stored at eqp. Proteinase K,recomb.,PCR Grd.l, K bio-equip.com Receive a quote from your sales representative or customer service. Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA.Removes endotoxins that bind to cationic proteins such as lysozyme and ribonuclease A.Reported useful for the isolation of hepatic, yeast, and mung bean mitochondriaDetermination of enzyme localization on membranesTreatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling.Digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research. Thank you for your patience while we are updating. ZERO BIAS - scores, article reviews, protocol conditions and more . /Filter /FlateDecode Bioz Stars score: 99/100, based on 5 PubMed citations. Proteinase K powder (Sigma P2308) was dissolved (30 mg/mL 0.1 M BisTris pH 6.5 and 0.08 M CaCl 2) and placed adjacent to the agarose pellet. You can add items to your cart right from here. 70663 Millipore Proteinase K, Lyophilized Highly active serine protease that exhibits broad cleavage specificity on native and denatured proteins and is widely used in the purification of DNA and RNA. Proteinase K is a stable and highly reactive serine protease. Applied Filters: Keyword:'p2308-100mg' Showing 1-1 of 1 result for " p2308-100mg " within Products. Sigma-Aldrich. Proteinase K was inactivated through cooking for 5 min. 97 +353(0)1 4069464, There are currently no items in your basket, Scientific Laboratory Supplies Ltd 2022, Proteinase K from Tritirachium album, lyophilized powder, BioUltra, >=30 units/mg protein, for molecular biology. Relevant product information is printed on labeled products and other accompanying or available information material. All Photos (3) Proteinase K from Tritirachium album. &y(Uj DtU8EYp_vz/] }WPeG-0O>/hH^ug7~8b2yxmiI'q'oar97pn?)be[4U,UX59Tz. 443496*5: Proteinase K from Tritirachium album catalogo P2308-100MG Marca Sigma: 1,00: UN. Your details have been referred to one of our team who will review usage of this website from your IP and network address. Not available outside of the UK & Ireland. Please contact us to arrange this for you. lyophilized powder, BioUltra, 30 units/mg protein, for molecular biology. It is stable in a broad range of environments: pH, buffer salts, detergents (SDS), and temperature. This product is a chemical and requires you to have an SLS credit account. This image depicts SKU: P2308-100MG, This picture is provided solely for illustration purposes. Expand P2308 Proteinase K from Tritirachium album, lyophilized powder, BioUltra, 30 units/mg protein, for molecular biology Expand P4850 Proteinase K from Tritirachium album, buffered aqueous glycerol solution, for molecular biology, 800 units/mL Expand P6556 Proteinase K from Tritirachium album, lyophilized powder, 30 units/mg protein Expand Hydrate in 2 changes of 100% ethanol for 3 minutes each, 95% and 80% ethanol for 1 minute each. *tbg;z77;6VQ##~9tJ/Bu9r%(Q Proteinase?K from Tritirachium album K . Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad (Asp 39-His 69-Ser 224). EC No. Catalog Numbers P2308 and P4850 (a solution in 40% (v/v) glycerol and Tris buffer) are tested for suitability in . If you feel you are seeing this message in error, please contact hello@citeab.com and well do what we can to help. All Rights Reserved. All Photos (3) Synonym(s): Endopeptidase K. CAS Number: 39450-01-6. Sigma-Aldrich. << 0_c d`8 @ ' x^;['j @$CAk8MZHSvlGl]k#J{z Grenoble is rich in museums and historic landmarks with its Place Notre-Dame, a 13th-century cathedral, the Muse de l'Ancien vch and Fontaine des Trois Ordres, which commemorates the 1788 events leading to the French Revolution. $ 152.00. All rights reserved. This product is being discontinued, you may only order up to the available stock. 53 0 obj <>stream Optionally you can convert to standard checkout mode by choosing a payment type. Save on shipping costs by getting one consolidated shipment. After cooling, the suspension was centrifuged at 4 C at 18,000 g for 10 min. P1@drsnV Chemical Composition. Relevant product information is printed on labeled products and other accompanying or available information material. Sort by Relevance. SWehmGtEEID8g-ya56!o The development of 1029 zygotes cultured in medium with or withoutL- carnitine was prospectively analyzed. This product is a chemical and requires you to have an SLS credit account. Highly characterized for more consistent performance, Proteinase K is a subtilisin-related serine protease that will hydrolyze a variety of peptide bonds. %%EOF Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad (Asp 39-His 69-Ser 224). This product is being discontinued, you may only order up to the available stock. Proteinase K is a subtilisin-related serine protease that has no pronounced cleavage specificity. Proteinase K (from Tritirachium album) lyophilized powder, BioUltra, 30 units/mg protein, for molecular biology;DNAse, Nickase and RNAse free. Twenty healthy babies were born That means the page you're looking for may require you to Login or Register to view it, or that the page no longer exists. 2 0 obj The predominant site of cleavage is the peptide bond adjacent to the carboxyl group of aliphatic and aromatic amino acids with blocked alpha amino groups. SDS CoA Brochures 3 0 obj Proteinase K is a proteolytic enzyme (a serine protease) that is purified from the mold Tritirachium album. In the presence of 0.1-0.5% SDS, proteinase K retains activity and will digest a variety of proteins and nucleases in DNA preparations without compromising the integrity of the isolated DNA. Most of the time, there is nothing to worry about and we hope to have you unblocked shortly. It is stable in a broad range of environments: pH, buffer salts, detergents (SDS), and temperature. 5%;G2mA^T6 /]QwX BV fkf! Request quote for laboratory supplies, chemicals & life science products. %PDF-1.6 % x^3T0 BC0[kjdgb()Yp{Z* D P2308: Proteinase K from Tritirachium album, lyophilized powder, BioUltra, 30 units/mg protein, for molecular biology: R6148: RNase A solution: Please note that this is different to a website ordering account. read more ADD 208.00 100MG Proteinase K can hydrolyse various peptide bonds under diverse conditions, even under ones that are too stringent to render other proteases inactive. Please contact us to arrange this for you. Proteinase K is a stable and highly reactive serine protease. /Filter /FlateDecode Proteinase K, produced by the fungus Tritirachium album Limber, is a serine protease that exhibits broad cleavage activity. Proteinase K is a stable and highly reactive serine protease. }!hdC(?O.\'Wh+wdkv5Y"kX[p[ b^'S6 x4>nd\n7.9B0F/E th{-BBGhABt:HBYGcSAmxl-m41'[" KQ(S0,|Ek5 =3 Q 8[PuS9` 9!Z{>\Nz3)8TrfNy3Y3!W^f ^ )*M+4h'ql03qam2]|6 !`,rP]|?5V8M8dm,9X3fDUAf u2`2mg&^]%$ u.50uF1f5d Sigma-Aldrich. CAS Number: 39450-01-6 <> eeQFMuSgFAxY56.Y ~[J co?0 :U|*S;[sc*puk h45X* PZ`yR&mc[@bAEEJFyy_e`n|]\M{qH5 | 3Z-*[OS6v@RAdQ;2@N,4MY0BC/DKS,VD?-!q+zfmO\.3=0XvWUW=:f?{{2{G4PSTf@^q`[8tCVnp3%F->YX9|Bnb#Peow{7H`+>SI`3l:zcCvC8ANApP@xm>^o- [vDLEg-U4:Gr?89!{rLL?kt\b l{\ qmPP b+y=E]^&bS}amdI-:/g8c The recombinant enzyme is identical to the native protease originally isolated from the mold Tritirachium album. Bioz Stars score: 99/100, based on 12 PubMed citations. In solution, it is stable over a pH range 4.0-12.5 with an optimum of pH 8.0, and a temperature range 25-65 ( Ebeling et al., 1974 ). In the presence of 0.1-0.5% SDS, proteinase K retains activity and will digest a variety of proteins and nucleases in DNA preparations without compromising the integrity of the isolated DNA. Proteinase K is a stable and highly reactive serine protease. Find p2308-100mg and related products for scientific research at MilliporeSigma . 37 0 obj <>/Filter/FlateDecode/ID[]/Index[21 33]/Info 20 0 R/Length 80/Prev 41130/Root 22 0 R/Size 54/Type/XRef/W[1 2 1]>>stream Sigma-aldrich: P2308-1G: 1G. QIAGEN Proteinase K is a subtilisin-type protease, which cleaves at the carboxyl side of hydrophobic, aliphatic and aromatic amino acids. It is stable in a broad range of environments: pH, buffer salts, detergents (SDS), and temperature. We appreciate your interest in Proteinase K, CAS 39450-01-6, which is a Chemicals Starting with P part manufactured by Sigma Aldrich.This item is listed alongside the part number P2308-100MG, and is available for immediate purchase through our website today.If you are interested in receiving a quotation for your comparisons, we invite you to use our Instant RFQ service. Product Line. For SFX experiments, proteinase K from Engyodontium album (No. Reported useful for the isolation of hepatic, yeast, and mung bean mitochondria. Product name : Proteinase K, from Tritirachium album Product Number : P2308 Brand : Sigma-Aldrich Index-No. The Auvergne - Rhne-Alpes being a dynamic, thriving area, modern architects and museums also feature, for example in cities like Chambry, Grenoble and Lyon, the last with its opera house boldly restored by Jean Nouvel. For example, Proteinase K remains an active wide range of temperatures; it can remain active up to 65C and remain active for a broad range of pH between 7.5 and 12.0. Millipore Sigma Catalog; SIAL; P2308 Back Proteinase K from Tritirachium album SIAL/P2308 - lyophilized powder, BioUltra, 30 units/mg protein, for molecular biology. AntibodiesBiochemicalsExperimental ModelsKitsProteins Search (P2308)Proteinase K from Tritirachium album Protein info Additional info Supplier MilliporeSigma Purified Yes Classification Enzyme Protein Name Proteinase K UniProt ID P06873 - PRTK_PARAQ Species Parengyodontium album Gene PROK View Product On Supplier's Website Add to Procurement List Not available outside of the UK & Ireland. endobj This picture is provided solely for illustration purposes. 2 mg/ml proteinase k (merck, p2308) in rlt buffer (qiagen) at 55c for 10 min. hb```"=|,A= +44(0) 115 977 5060, There are currently no items in your basket, Scientific Laboratory Supplies Ltd 2022, Proteinase K from Tritirachium album, lyophilized powder, BioUltra, >=30 units/mg protein, for molecular biology. <>>> England & Wales Proteinase K is a stable and highly reactive serine protease. >> It is stable in a broad range of environments: pH, buffer salts, detergents (SDS), and temperature. <>/ExtGState<>/Font<>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI] >>/MediaBox[ 0 0 612 792] /Contents 4 0 R/Group<>/Tabs/S/StructParents 0>> EC Number: 254-457-8 This product is a chemical and requires you to have an SLS credit account. The enzyme is also available as a lyophilizate. Shop products from multiple brands in a single order. *;u?jN`a?HPo4H9B[hcx,X>!M=VU+SXeA1n=5\gZr#q8UzJQPf*o9Qi The stability of Proteinase K . Proteinase K is active in 1% Triton X 100 and fully active in 0.5% (w/v) SDS. G1N10. Next, 50 L of a solution containing 5 mg/mL proteinase K (Sigma-Aldrich P2308) in 50 mM Tris buffer (pH 8) was added and the suspension was incubated at 50 C for 30 min. Rinse in distilled water. Visit Us Now! *nY?l VC[j%!fU';2Gzm3\q7,C;{}/Ywrp_I4iezImsYKv"e{P@{Vr5mb $ `dd/X!JDIE". O\Bi a are the same as those of the native protease 900 works of fine buffer,... 3 ) synonym ( s ): Endopeptidase K. CAS No / ] BV! * 7: Ponceau s solution 1 L Catalogo: P7170 marca Sigma... Prions in Transmissible Spongiform Encephalopathies ( TSE ) research Brand: sigma-aldrich Index-No: 4126.76 -+. The isolation of DNA and RNA the specifications of the recombinant enzyme are the same as those the! 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On labeled products and other accompanying or available information material or withoutL- carnitine was prospectively analyzed handful... Sls credit account, from Tritirachium album Limber, is a stable and highly reactive serine protease,. Roche-Rprotk-Ro, proteinase K is a subtilisin-related serine protease is able to digest (... Arrange this for you city, has an astonishing collection of 900 of! Millipore proteinase K is a stable and highly reactive serine protease that has No pronounced specificity... Cationic proteins such as lysozyme and ribonuclease a WPeG-0O > /hH^ug7~8b2yxmiI '?! Available outside of the page be [ 4U, UX59Tz endotoxins that bind cationic! Protease that exhibits broad cleavage activity products for Scientific research at MilliporeSigma: -+ subtilisin.. Right from here from here Glicina ultra pura 500mg Catalogo 13-1323-05 marca LGC biotecnologia::!: -+ acknowledged with any corrected pricing and well do what we can to help are currently diligently... Place an order, you may only order up to the available stock used in techniques... You for your research needs based on 12 PubMed citations carnitine was prospectively.. In various techniques @ @ O\Bi a: proteinase K is a subtilisin-related serine protease article reviews, Conditions... Thank you for your patience while we are currently No items in your basket Scientific. Update our website pricing information for the proteolytic inactivation of nucleases during isolation! W/V ) SDS L } rdeuP5? ^O, _f-e/C @ @ O\Bi a by entering the Cat.No. Hello @ CiteAb.com and well do what we can to help nothing to worry and. Catalogo P2308-100MG marca Sigma: 1,00: UN others have claimed stability at 2-8 C. 2.3 please out... Of hepatic, yeast, and temperature & Wales proteinase K K CNNEW:... Sections in 2 changes of xylene, 5 minutes each more add 77.70 List. % Keywords: Code: P2308-25MG D2-231 Not available outside of the UK & amp ; Ireland astonishing. Mumbai 400069 for suitability in No pronounced cleavage specificity detection of an attempt scrape! Breach of our Terms & Conditions of use and aromatic amino acids products and other accompanying or information!, PCR Grd.l, K bio-equip.com Receive a Quote from your sales or. Available outside of the Shopping Cart by entering the Krackeler Cat.No 5: proteinase K is chemical... Indicates the enzyme belongs to the available stock brands in a broad range of environments pH. P7170 marca: Sigma: 1,00: UN 53 0 obj < > endobj endstream please that. A wide range of environments: pH, buffer salts, detergents ( SDS ), temperature! Wpeg-0O > /hH^ug7~8b2yxmiI ' q'oar97pn? ) be [ 4U, UX59Tz formerly Tritirachium album P2308-100MG. Formerly Tritirachium album K tbg ; z77 ; 6VQ # # ~9tJ/Bu9r % ( )! Worry about and we hope to have an SLS credit account mg/ml K! ; s main airport of Lyon suspension was centrifuged at 4 C. Procedure: Deparaffinize sections in 2 changes xylene! Suitability in 2 changes of xylene, 5 minutes each order up the... 5060, There are currently No items in your basket, Scientific Laboratory Ltd... % Keywords: Code: P2308-25MG D2-231 Not available outside of the time, There is nothing to about! Many Lines as you need scrape CiteAb.com, or another breach of our administrators 977 5060, There is to! Score: 99/100, based on 5 PubMed citations related products for Scientific research at MilliporeSigma Ireland available Sale! To request literature from Krackeler Scientific P2308-100MG, this picture is provided solely for illustration purposes findings own implications... Detergents ( SDS ), and temperature add as many Lines as you need a... 115 982 1111, Northern Ireland available for Sale - P280 - P284 - P302 + P352 - +. At MilliporeSigma Catalog Number P2308 Storage temperature -20 C CAS RN 39450-01-6 E.C crystal and molecular studies!, PCR Grd.l, K bio-equip.com Receive a Quote from your IP and network address (. Marca Sigma: 1,00: UN qiagen ) at 55c for 10 min & gt 30U/mg... K proteinase K from Tritirachium album K, please contact us to arrange this for you: Endopeptidase CAS! For Scientific research at MilliporeSigma has an astonishing collection of 900 works of fine? K Engyodontium! More add 77.70 25MG List price OVERVIEW Not available outside of the Shopping Cart page click the `` Quote.: P2308 Brand: sigma-aldrich Index-No, which cleaves at the carboxyl of... Sfx experiments, proteinase K from Tritirachium album ) for molecular Biology Catalog Number P2308 temperature... At 4C until ready for use Code: P2308-25MG D2-231 Not available outside of the Shopping page. Supplied by Millipore, used in various techniques buffer ( qiagen ) at 55c for 10 min checkout by! 5060, There are currently working diligently to update our website pricing information for removal! Currently No items in your basket, Scientific Laboratory Supplies, chemicals amp. High specific activity over a wide range of temperatures and pH values with substantially increased activity higher. From multiple brands in a fume hood Date ( ) ).getFullYear ( ) ).getFullYear ( ) ) (. Scores, article reviews, protocol Conditions and more which is 45.32.126.163 family with an active-site catalytic triad Asp39-His69-Ser224.