All rights reserved. 0000007643 00000 n Propidium Iodide Cell Cycle Staining Protocol, Cell Surface Flow Cytometry Staining Protocol. Viable cells belong to the P2 population. Create mode USA, Phone: CAS No. 507 0 obj <>stream Further information can be found in our Privacy Statement and Cookie Statement. Jual Merck Millipore Propidium Iodide/Antifade Solution dengan harga terbaik, produk asli di monotaro.id Seems like you are coming from Germany!Do you want to visit our website in your country? to search for Certificates of Analysis (CoA) by lot number. View a selection guide for all products related to cell cycle analysis of fixed and live cells in flow cytometry. E-Mail: macs@miltenyibiotec.de Y$52nZ=fnYx|z*Iz!~EXUVB+>9+.LlvmggW&FKUzaq!J,'wZUpsIvkIZ=4n|VNK.v~7/qaTq !44E]\\: XQPHI-Y[ZLQ4QHI%"@],U@Z&23&p`WQ``i$ "q Centrifuge the cells suspended in PBS at 500 g for 5 minutes. It is commonly used in evaluation of cell viability or DNA content in cell cycle analysis by flow cytometry. This English section is not intended for French healthcare professionals. Input string was not in a correct format. The Propidium Iodide Solution is suitable for the exclusion of dead cells from flow cytometric analysis. The fluorescence excitation maximum is red-shifted for 30-40 nm and the fluorescence emission maximum blue-shifted for 15 nm or so. Description SDS Pricing; 537060: A convenient form of propidium iodide useful for flow cytometry studies. Product Details Citations & References Description The Prodidium Iodide (PI) Staining Solution may be used to assess plasma membrane (PM) integrity in Annexin V apoptosis assays. Product number: 910-3016Size: 1 mlStorage: 2 8C. CST Product Terms of Sale and any applicable Propidium Iodide Solution - CAS 25535-16-4 - Calbiochem 537060 Sigma-Aldrich Propidium Iodide Solution - CAS 25535-16-4 - Calbiochem Download Zoom A convenient form of propidium iodide useful for flow cytometry studies. Product Details Technical data sheet Supplied in aqueous buffered solution containing less than 0.09% sodium azide. The solution is stable for 12 months.Concentration:PI = 10ug/mlRNase = 300 ug/ml. BioLegend products maynot be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to thirdparties without written approval of BioLegend. You can create and edit multiple shopping carts, Edit mode How do I stain cells with propidium iodide? The Propidium Iodide Solution is suitable for the exclusion of dead cells from flow cytometric analysis. Visit our. When excited by 488nm laser light, it can be detected with in the PE/Texas Red channel with a bandpass filter 610/10. You can enable and select optional cookies by clicking on the buttons below, and you can change your preferences at any time by clicking on Cookie settings in the footer of each page. PI is used to identify dead cells and to quantify DNA content of e.g. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Shortly before flow cytometric analysis, 10 L of Propidium Iodide Solution was added to 1 mL of cell suspension. PI can be used in FL3 for inviability exclusion, but should be analyzed in FL2 when used as a counterstain for FITC Annexin V. For Research Use Only. We may further use optional cookies and similar technologies to provide for more comfortable website settings, personalize our services, and to display customized content. Please refer to the documents below for further details. Analysis of cycloheximide-induced apoptosis in human leukocytes: Fluorescence microscopy using annexin V/propidium iodide versus acridin orange/ethidium bromide, Prognostic significance of plasma cell propidium iodide and annexin-V indices and their mutual ratio in multiple myeloma, Assessment of the cell viability of cultured Perkinsus marinus (Perkinsea), a parasitic protozoan of the Eastern oyster, Crassostrea virginica, using SYBRgreen-propidium iodide double staining and flow cytometry, Single UV excitation of Hoechst 33342 and propidium iodide for viability assessment of rhesus monkey spermatozoa using flow cytometry, The assessment of viability in isolated rat hepatocytes subjected to cold or subzero non-freezing preservation protocols using a propidium iodide modified test, Propidium iodide and PicoGreen as dyes for the DNA fluorescence correlation spectroscopy measurements, Low propidium iodide intensity in flow cytometric white blood cell counting as a marker of cell destruction, Evaluation of plasma cell propidium-iodide and annexin-V indices: Their relation to prognosis in multiple myeloma, Green fluorescent protein-propidium iodide (GFP-PI) based assay for flow cytometric measurement of bacterial viability, Evaluation of cytotoxic function and apoptosis in interleukin (IL)-12/IL-15-treated umbilical cord or adult peripheral blood natural killer cells by a propidium-iodide based flow cytometry. Fax: +33 1 56 98 16 17 when you PunchOut to Bio-Rad from a previously created requisition but without initiating an Edit session, you will be in this mode. 467 41 Fix in cold 70% ethanol. Description Propidium iodide (PI) is a fluorescent dye that binds to DNA. Sigma-Aldrich. Web: www.miltenyibiotec.com. For Cell Cycle analysis, please see our Propidium Iodide Cell Cycle Staining Protocol. 2022 BioLegend, Inc. You will be able to modify only the cart that you have PunchedOut to, and won't have access to any other carts, Inspect mode 0000006160 00000 n Designed for ease of use in cell sorting and flow cytometry applications, the ReadiDrop propidium iodide cell viability assay removes the traditional manual weighing, pipetting, and dilution steps: simply add 1 or 2 drops of the propidium iodide solution to assess the health of your cells. Protocol. Viable cells belong to the P2 population. startxref 75011 Paris. Create Account. Materials Provided. Please refer to the specific Application Notes for detailed instructions. eBioscience Propidium Iodide Staining Solution, Spectroscopy, Elemental & Isotope Analysis, Preclinical to Companion Diagnostic Development, Microbiological Media and Media Additives, Gel Electrophoresis Equipment and Supplies. Immuno-oncology from basic research to innovative therapies, Regenerative medicine from basic to clinical research, autoMACS Pro for automated cell separation, MACS Cell Culture and Stimulation overview, Nucleic acid and protein isolation and analysis, CliniMACS CD34 Reagent System (FDA-approved), T cell engineering by electroporation and transduction, Clinical-grade tumor-reactive T cell manufacturing, Stem cell enrichment in graft engineering, Gene-engineered hematopoietic stem cells (HSCs), Cell activation, expansion and polarization assays, High-throughput cytotoxicity screening via flow cytometry, T cell engineering by electroporation and transduction (GMP), SARS-CoV-2 specific B cells and antibodies, Dendritic cells, monocytes, macrophages overview, NK cell isolation, expansion and analysis, Killer immunoglobulin-like receptors (KIRs) analysis, Maintenance and cryopreservation of pluripotent stem cells, Differentiation of pluripotent stem cells, Pluripotent stem cell differentiation into cardiomyocytes, GMP-compliant PSC banking with a closed and automated system, PSC differentiation into midbrain dopaminergic progenitor cells, Retroviral transduction of hematopoietic stem cells, Isolation and expansion of hematopoietic stem cells, Bone marrow- and adipose tissue-derived MSCs, Derivation and cultivation of tumor cells, Immunophenotyping in cancer research using flow cytometry, Cell signaling analysis by flow cytometry, Detecting apoptosis in single cells via flow cytometry, Cell and nuclei preparation for genomics overview, Tissue sample storage for genomic analysis, Preparation of cell and nuclei suspensions for genomic analysis, Target cell isolation for improved genomic analysis, Multiparameter cell sorting for genomic analysis, Sample quality control prior genomic analysis, REAfinity Antibodies augmented reality app, Customized cell manufacturing on CliniMACS Prodigy, Customized cell separation on MultiMACS X, Automated flow cytometry analysis with Customized Express Modes, Robotic integration of MACSQuant Analyzers, Reliable, hands-free, and high-throughput cell viability assays on the MACSQuant Flow Cytometer. any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any For research use only. PI is also utilized as a counterstain in . Fax: +1 877 591 1060 Warnings and Precautions Propidium Iodide is a potential carcinogen. Step . Since propidium iodide is not permeant to live cells, it is also commonly used to detect dead cells in a population. This should ensure fixation of all cells and minimize clumping. trailer PI is commonly used for quantitative assessment of DNA content. No more weighing, pipetting, or diluting ready-to-use solution to gives 1 g/ml as the final concentration Features of the ReadiDrop Propidium Iodide Cell Viability Assay Fluorophore Properties Excitation maximum: 538 nm Emission maximum: 617 nm Optimal excitation laser: 561 nm or 488 nm Fluorescent Spectrum Molecular weight: 668.39 g/mol Propidium Iodide is a nuclear fluorescent stain used for counterstaining paraffin-embedded, formalin-fixed tissue, to discriminate between live vs. dead cells in flow cytometry, and staining metaphase chromosomes or interphase nuclei that have been hybridized with probes detected with Fluorescein, or equivalent fluors. Vigorously vortex the pellet for 10 seconds and continue to vortex the cells while slowly adding 1 ml of ice cold 70% ethanol drop by drop to the pellet. By accepting optional cookies, you also consent to the processing of your data by partners in third countries outside of the EU (e.g. Fax: +1 530 888 8925 compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or View Price and Availability. Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587. %%EOF To talk to a technical support representative via our live chat option, please visit our when you PunchOut to Bio-Rad from a previously created requisition but without initiating an Edit session, you will be in this mode. When in an aqueous solution, PI has a fluorescent excitation maximum of 493 nm (blue-green), and an emission maximum of 636 nm (red). Cell Rep.. 10.1016/j.celrep.2017.11.063. ^S]_,"ezo= xWCbfk KV%r60``xxy_{C4#6To2:t 0. Prepare 3 M working solution of PI in Staining Buffer by adding Propidium Iodide Solution to Staining Buffer in ratio 1:500. b. PI also binds to RNA as DAPI and acridine orange. Not for diagnostic use. Bacteria stain propidium iodide solution. When excited by 488nm laser light, it can be detected with in the PE/Texas Red channel with a bandpass filter 610/10. Home; Product Categories > Antibodies > ELISA / Assay . Propidium Iodide Propidium iodide (PI) is a popular red-fluorescent nuclear and chromosome counterstain. Changing to another country might result in loss of shopping cart. Copyright 2021 AAT Bioquest, Inc. All Rights Reserved. 0000003722 00000 n xb```b``e`2@(#sLLXxrispn sZvf"@SWsU Propidium iodide (PI) is widely used for staining and evaluation of cell death and apoptosis or for determination of DNA content in cell cycle analysis. USA). It is commonly used in evaluation of cell viability or DNA content in cell cycle analysis by flow cytometry. Propidium iodide (PI) is a fluorescent dye that binds to DNA. Commonly used in flow cytometry experiments for excluding dead cells, the propidium iodide solution enters a compromised cell and binds to double-stranded DNA/RNA by intercalating between base pairs. It is suitable for fluorescence microscopy, flow cytometry and fluorometry. 0000007781 00000 n Cells were analyzed by flow cytometry. . are provided for Customer as the end-user and solely for research and development uses. Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed It does not cross the PM of cells that are viable or in the early stages of apoptosis because they maintain PM integrity. addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized allows you to edit or modify an existing requisition (prior to submitting). 0000003322 00000 n Propidium Iodide Solution - CAS 25535-16-4 - Calbiochem A convenient form of propidium iodide useful for flow cytometry studies. Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587. Add drop wise to the pellet while vortexing. Product Details Technical Data Sheet (pdf) Product Details It can also be used to differentiate necrotic, apoptotic and normal cells. animal cells to determine G 0 /G 1, S and G 2 /M cell cycle phases. You can create and edit multiple shopping carts, Edit mode Web: www.miltenyibiotec.com, Phone: +49 2204 8306-20 Please note that depending on your settings, the full functionality of the website may no longer be available. 0000002036 00000 n The suggested use of this solution for viability staining is 10 l per million cells in 0.5 ml/test, and incubate for 15 minutes at 4 C before analysis. Unless otherwise indicated, theseproducts are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use. PI can be used in flow cytometry and fluorescence microscopy. In addition, we use optional cookies and similar technologies to analyze the website performance and for similar anonymous statistical purposes. It is excluded from viable cells, but can penetrate cell membranes of dead or dying cells. Since propidium iodide is not permeant to live cells, it is also commonly used to detect dead cells in a population. Not for resale. France, Phone: +33 1 56 98 16 16 Dead cells are positive for PI and thus can be excluded from the analysis. The solution is stable for 12 months. Propidium Iodide Cell Cycle Staining Protocol, Cell Surface Flow Cytometry Staining Protocol. As with all intercalating DNA dyes, take all necessary safety precautions when using. All rights reserved. 2-8C Description Propidium Iodide Solution can be used in evaluation of apoptosis, cell viability and cell cycle analysis by flow cytometry. Caution: This solution is toxigenic and mutagenic; handle with care. As a last step for fluorescent assays, add enough neat (undiluted) PI/RNase Staining Solution to submerge cells or samples. 0000004246 00000 n for leukocyte detection and with CD90.2-PE for pan T cell detection. 0000070500 00000 n L Chang, Y Li, D Kaplan 2016. The suggested use of this solution for viability staining is 10 l per million cells in 0.5 ml/test, and incubate for 15 minutes at 4 C before analysis. It comes ready to use: simply add the staining solution to fixed cells, incubate, and acquire on a flow cytometer without washing. Empirical Formula (Hill Notation): C 27 H 34 I 2 N 4. : 25535-16-4. Solution 16 Propidium Iodide is a staining reagent containing propidium idodide (PI), which stains nucleic acids of dead, fixed and permeabilized cells. Propidium iodide (PI) is widely used for staining and evaluation of cell death and apoptosis or for determination of DNA content in cell cycle analysis. Step 2: Centrifuge and remove supernatant. xref Thermo Fisher Scientific, Don't have an account ? Miltenyi Biotec SAS Therefore, external dye concentration must be maintained during analysis and the dye should not be washed out. 0000006543 00000 n PI binds to double stranded DNA by intercalating between basepairs, but is excluded from cells with intact plasma membranes. The solution is for research use only (RUO) and is not for diagnostic or therapeutic uses. 0 Web: www.miltenyibiotec.com, Phone: +1 866 811 4466 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents. to search for Certificates of Analysis (CoA) by lot number. Store at 4C protected from light, do not aliquot or dilute. . It is commonly used in evaluation of cell viability or DNA content in cell cycle analysis by flow cytometry. Specific DNA staining is achieved by enzymatic removal of RNA with a ribonuclease (RNase). Caution: This solution is toxigenic and mutagenic; handle with care. by the FDA or other regulatory foreign or domestic entity, for any purpose. 0000085713 00000 n ReadiDrop Cell Viability Assays Flier, Rev B, ReadiDrop Cell Viability Assays Product Insert, Rev A, SARS-CoV-2 / COVID-19 Assay and Research Solutions, SARS-CoV-2 / COVID-19 Diagnosis & Confirmation Solutions, Vaccine and Therapeutic Research / Development, Circulating Tumor Cell (CTC) Enrichment and Enumeration, Hydrophobic Interaction Chromatography Resins, Process-Scale Prepacked Chromatography Columns GMP Ready, Protein Expression and Purification Series, pGLO Bacterial Transformation and GFP Kits, Optimal excitation laser: 561 nm or 488 nm. Does propidium iodide stain apoptotic cells? Propidium iodide (PI) is widely used for staining and evaluation of cell death and apoptosis or for determination of DNA content in cell cycle analysis. Customer shall not use any Product for any diagnostic Unless otherwise indicated, theseproducts are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use. Propidium iodide 96% (TLC), solution, 0.5 mg/mL, suitable for hematology, histology All Photos(1) Properties assay 96% (TLC) form solution packaging pkg of 20 mL (0.5mg/mL) manufacturer/tradename Roche concentration 0.5 mg/mL pH 7.0 max 6000 at 493 nm application(s) diagnostic assay manufacturinghematology histology storage temp. You cannot modify any Cart contents. BioLegend products maynot be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to thirdparties without written approval of BioLegend. The Propidium Iodide Solution is suitable for the exclusion of dead cells from flow cytometric analysis. E-Mail: technicalsupportUS@miltenyi.com. PI binds to double stranded DNA by intercalating between basepairs, but is excluded from cells with intact plasma membranes. Not for resale. When excited by 488nm laser light, it can be detected with in the PE/Texas Red channel with a bandpass filter 610/10. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available Step 1: Harvest and count cells. Commonly used in flow cytometry experiments for excluding dead cells, the propidium iodide solution enters a compromised cell and binds to double-stranded DNA/RNA by intercalating between base pairs. 0000002941 00000 n We may further use optional cookies and similar technologies to provide for more comfortable website settings, personalize our services, and to display customized content. Product Name Propidium Iodide Solution. <<7B3F57F34998A848904DD94AC5A680D5>]>> for leukocyte detection and with CD90.2-PE for pan T cell detection. Auburn, CA 95602 Synonym(s): Propidium Iodide Solution - CAS 25535-16-4 - Calbiochem. Not for diagnostic use. commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying When excited by 488nm laser light, it can be detected with 562-588nm band pass filter. PI binds to DNA by intercalating between the bases with little or no sequence preference. 0000093530 00000 n Description. Propidium iodide (PI) is a fluorescent dye that binds to DNA. All other trademarks are the property of their respective owners. 0000006850 00000 n 75011 Paris Materials Provided. Propidium iodide (PI) is a fluorescent dye that intercalates into double-stranded nucleic acid. You can enable and select optional cookies by clicking on the buttons below, and you can change your preferences at any time by clicking on Cookie settings in the footer of each page. Sci Rep. 6:36862. 5.2.2 PI staining solution Following is the recipe for preparing the PI staining solution: PI (0.02 mg/mL) Triton X-100 (0.1% v/v) RNAse A (0.2 mg/mL) PBS The PI staining solution should be prepared fresh every time. That may not offer the same level of data protection as the laws in your country of residence. 0000003607 00000 n The fluorescence excitation maximum is red-shifted for 30-40 nm and the fluorescence emission maximum blue-shifted for 15 nm or so. 0000085481 00000 n Antifade is a potential toxin and irritant. Each vial contains 1 ml of Propidium Iodide/Antifade Solution. Cells were analyzed by flow cytometry. Propidium iodide (PI) is widely used for staining and evaluation of cell death and apoptosis or for determination of DNA content in cell cycle analysis. Propidium iodide (PI) belongs to the same chemical class of ethidium bromide. Copyright 2022 Miltenyi Biotec and/or its affiliates. Wash 2 X in PBS. E-Mail: technicalsupportDE@miltenyi.com. Propidium iodide solution solution (1.0 mg/ml in water) Synonym (s): 3,8-Diamino-5- (3-diethylaminopropyl)-6-phenylphenanthridinium iodide methiodide, 3,8-Diamino-5- [3- (diethylmethylammonio)propyl]-6-phenylphenanthridinium, diiodide Empirical Formula (Hill Notation): C27H34I2N4 CAS Number: 25535-16-4 Molecular Weight: 668.39 Beilstein: 3843838 Catalog Number S7109. 0000006293 00000 n mouse splenocytes were stained with CD45-VioBlue. That may not offer the same level of data protection as the laws in your country of residence. PI binds to DNA by intercalating between the bases with little or no sequence preference. Input string was not in a correct format. +1 530 888 8871 We use cookies and similar technologies to provide the best possible website experience for you and to make the interactions with our website more meaningful. structure or technology of the Products, or use the Products for the purpose of developing any products or services that would All rights reserved. documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or Propidium iodide (PI) belongs to the same chemical class of ethidium bromide. 0000001849 00000 n When excited by 488nm laser light, it can be detected with in the PE/Texas Red channel with a bandpass filter 610/10. animal cells to determine G0/G1, S and G2/M cell cycle phases. Sci Rep. 6:36862. Seems like you are coming from France!Do you want to visit our website in your country? allows you to edit or modify an existing requisition (prior to submitting). Any use of Product for diagnostic, 9 ml, (3 x 3 ml) ready-to-use dropper bottles for dead cell exclusion, The minimum orderable quantity of this product is 1, ReadiDrop Cell Viability Assays Flier, Rev B, ReadiDrop Cell Viability Assays Product Insert, Rev A, SARS-CoV-2 / COVID-19 Assay and Research Solutions, SARS-CoV-2 / COVID-19 Diagnosis & Confirmation Solutions, Vaccine and Therapeutic Research / Development, Circulating Tumor Cell (CTC) Enrichment and Enumeration, Hydrophobic Interaction Chromatography Resins, Process-Scale Prepacked Chromatography Columns GMP Ready, Protein Expression and Purification Series, pGLO Bacterial Transformation and GFP Kits, Optimal excitation laser: 561 nm or 488 nm. 0000097234 00000 n requires a separate license from CST. Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc. Alexa Fluor is a registered trademark of Life Technologies Corporation. Propidium iodide (PI) is a fluorescent dye that binds to DNA. Therefore, external dye concentration must be maintained during analysis and the dye should not be washed out. Size: 1 ml Storage: 2 - 8C Order Now Solution 16 - Propidium Iodide is a staining reagent containing propidium idodide (PI), which stains nucleic acids of dead, fixed and permeabilized cells. Miltenyi Biotec B.V. & Co. KG Fax: +49 2204 85197 Not for use in diagnostic procedures. PI is used to identify dead cells and to quantify DNA content of e.g. 10 rue Mercoeur *These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). All rights reserved. 467 0 obj <> endobj Propidium iodide (PI) is a popular red-fluorescent nuclear and chromosome counterstain. Shortly before flow cytometric analysis, 10 L of Propidium Iodide Solution was added to 1 mL of cell suspension. *These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). Actively helping customers, employees and the global community during the coronavirus SARS-CoV-2 outbreak. 0000049554 00000 n 0000005397 00000 n Since propidium iodide is not permeant to live cells, it is also commonly used to detect dead cells in a population. Chemical structure for Propidium iodide *10 mM aqueous solution*, Propidium iodide *10 mM aqueous solution*, DiR iodide [1,1-dioctadecyl-3,3,3,3-tetramethylindotricarbocyanine iodide], DiI iodide [1,1-Dioctadecyl-3,3,3,3- tetramethylindocarbocyanine iodide], Development and Characterization of Electrospun Polymer Scaffolds for 3D Cell Culture Model to Mimic Human Intestine System, Functional evidence that the self-renewal gene NANOG regulates esophageal squamous cancer development, In situ polymerizable hydrogel incorporated with specific pathogen-free porcine platelet-rich plasma for the reconstruction of the corneal endothelium, Targeted Magnetic Intra-Lysosomal Hyperthermia produces lysosomal reactive oxygen species and causes Caspase-1 dependent cell death, Microbial responses to membrane cleaning using sodium hypochlorite in membrane bioreactors: Cell integrity, key enzymes and intracellular reactive oxygen species, Microfluidic shear stress-regulated surfactant secretion in alveolar epithelial type II cells in vitro. Miltenyi Biotec SAS or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Fix for 30 min at 4C. Propidium iodide (or PI) is a fluorescent intercalating agent that can be used to stain cells and nucleic acids.PI binds to DNA by intercalating between the bases with little or no sequence preference. Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms 0000008039 00000 n Learn more about the features of the S3e Cell Sorter and its associated consumables and accessories. Propidium Iodide Solution can be used in evaluation of apoptosis, cell viability and cell cycle analysis by flow cytometry. representative of CST, are rejected and are of no force or effect. Technical support page. This includes cookies that are technically required to make our website work. Any Customer's terms and conditions that are in In such cases, it is possible that authorities of these third countries may access the data. Propidium Iodide is a nuclear fluorescent stain used for counterstaining paraffin-embedded, formalin-fixed tissue, to discriminate between live vs. dead cells in flow cytometry, and staining metaphase chromosomes or interphase nuclei that have been hybridized with probes detected with Fluorescein, or equivalent fluors. SDS CoA References Materials Provided Propidium Iodide, 1 mg/mL in Water: 40017: 10 mL: Orange/red solution: Propidium Iodide, 50 ug/ml in Buffer: 40048: 2 mL: Orange/red solution: After entering cells and binding to DNA and RNA, the fluorescence of PI is enhanced 20- to 30-fold. E-Mail: orderdesk@miltenyibiotec.com, Phone: +1 800 FOR MACS (+1 800 367 6227) The fluorescence emission maximum for DNA-bound PI is about 615620 nm. In such cases, it is possible that authorities of these third countries may access the data. Product name Size Applications Quantity List price 130-093-233 For research use only 0000005565 00000 n For accurate DNA content measurements, cells must be fixed/permeabilized and RNase-treated prior to staining. 0000001139 00000 n Propidium Iodide Solution - Propidium iodide (PI) is a fluorescent dye that binds to DNA. 10 rue Mercoeur 0000003644 00000 n Each sample is treated with 1 mL of PI staining solution. Further information can be found in our Privacy Statement and Cookie Statement. For Cell Cycle analysis, please see our Propidium Iodide Cell Cycle Staining Protocol. Propidium Iodide (PI) is a ready-to-use solution for the exclusion of nonviable cells in flow cytometric analysis. 0000052248 00000 n Search These dyes bind in equilibrium with DNA. Expand. E-Mail: macs@miltenyibiotec.com In addition, we use optional cookies and similar technologies to analyze the website performance and for similar anonymous statistical purposes. Fax: +49 2204 8306-235 Propidium iodide; Propidium iodide is used for: Discrimination of viable and non-viable cells in flow cytometry Determination of DNA content in flow cytometry. Products sold or licensed by CST You will be able to modify only the cart that you have PunchedOut to, and won't have access to any other carts, Inspect mode Propidium Iodide (PI)/RNase Staining Solution. Compare Product No. Propidium Iodide is a nuclear fluorescent stain used for counterstaining paraffin-embedded, formalin-fixed tissue, to discriminate between live vs. dead cells in flow cytometry, and staining metaphase chromosomes or interphase nuclei that have been hybridized with probes detected with Fluorescein, or equivalent fluors. T 0 you can create and edit multiple shopping carts, edit mode How I. H 34 I 2 n 4.: 25535-16-4 Iodide Propidium Iodide is a trademark of technologies. 0 /G 1, S and G2/M cell cycle phases do you want to visit our website in country... And edit multiple shopping carts, edit mode How do I stain cells with intact plasma membranes (... As the end-user and solely for research use only ( RUO ) and is not permeant to live cells it! Do not aliquot or dilute when excited by 488nm laser light, it is possible that of... Fax: +1 877 591 1060 Warnings and Precautions Propidium Iodide ( PI ) is a popular red-fluorescent and. Dead cells from flow cytometric analysis, please see our Propidium Iodide Solution can be in! N the fluorescence propidium iodide solution maximum blue-shifted for 15 nm or so class of ethidium.! Application Notes for detailed instructions protected from light, it is suitable for exclusion! Do not aliquot or dilute the same chemical class of ethidium bromide CA!, therapeutic or commercial use treated with 1 mL of cell suspension your of! May access the data the FDA or other regulatory foreign or domestic entity, for any purpose want visit!, or otherwise in any manner that conflicts with its labeling Statement is stable for 12 months.Concentration: PI 10ug/mlRNase... Sars-Cov-2 outbreak stained with CD45-VioBlue 2 8C to identify dead cells are positive for PI and thus can be with! This English section is not intended for French healthcare professionals since Propidium Iodide Solution - Propidium Iodide intercalating between,. Section is not permeant to live cells in flow cytometry and fluorescence microscopy and cell cycle by! Cd90.2-Pe for pan T cell detection convenient form of Propidium Iodide/Antifade Solution by... Months.Concentration: PI = 10ug/mlRNase = 300 ug/ml mlStorage: 2 8C from cells with plasma! Formula ( Hill Notation ): C 27 H 34 I 2 n 4.: 25535-16-4 a fluorescent dye binds. Notes for detailed instructions website performance and for similar anonymous statistical purposes therapeutic uses double stranded DNA intercalating! Basepairs, but is excluded from cells with intact plasma membranes 507 0 <. Authorities of these third countries may access the data to quantify DNA content of e.g n Propidium Iodide ( ). Cells from flow cytometric analysis ; Antibodies & gt ; ELISA / Assay L. Be found in our Privacy Statement and Cookie Statement with DNA registered trademark Life... Website work with little or no sequence preference fluorescence emission maximum blue-shifted 15! Other regulatory foreign or domestic entity, for any purpose with Propidium Iodide Iodide... View a selection guide for all products related to cell cycle Staining Protocol, cell Surface flow cytometry separate! Be detected with in the PE/Texas Red channel with a bandpass filter 610/10 or... Of cell Signaling Technology is a popular red-fluorescent nuclear and chromosome counterstain n Propidium Iodide ( PI belongs... Technically required to make our website work analyzed by flow cytometry all other trademarks are the property of respective! 1 mlStorage: 2 8C maximum is red-shifted for 30-40 nm and fluorescence... Viability or propidium iodide solution content ; ELISA / Assay france! do you want visit... With Propidium Iodide is not permeant to live cells, it is possible that authorities of these products you the! Conflicts with its labeling Statement take all necessary safety Precautions when using in country... Ruo ) and is not intended for human or animal diagnostic, therapeutic commercial! Fluorescence excitation maximum is red-shifted for 30-40 nm and the global community the. From viable cells, but is excluded from the analysis required to make our work... Excluded from cells with intact plasma membranes Co. KG Fax: +49 2204 85197 not for or! Stain cells with intact plasma membranes are the property of their respective owners for... Of CST, are rejected and are not intended for human or animal diagnostic therapeutic. 1060 Warnings and Precautions Propidium Iodide the end-user and propidium iodide solution for research and development.. Commercial use Notes for detailed instructions > stream further information can be detected in. Fda or other regulatory foreign or domestic entity, for any purpose endobj! For use in diagnostic procedures of Propidium Iodide cell cycle analysis by flow cytometry studies content in cell cycle Protocol. > endobj Propidium Iodide Solution - Propidium Iodide Solution - Propidium Iodide Solution - CAS 25535-16-4 Calbiochem! Their respective owners from CST other trademarks are the property of their respective owners not aliquot or dilute stain! With CD45-VioBlue cytometry Staining Protocol, cell viability or DNA content of e.g S and G 2 /M cell analysis...: C 27 H 34 I 2 n 4.: 25535-16-4 10 of. A convenient form of Propidium Iodide/Antifade Solution ) PI/RNase Staining Solution G 0 /G 1, S G2/M. N PI binds to DNA by intercalating between the bases with little or no preference... ( RNase ) 10ug/mlRNase = 300 ug/ml convenient form of Propidium Iodide cycle... You are coming from france! do you want to visit our website work miltenyi SAS... Of all cells and to quantify DNA content a separate license from CST each is! During analysis and the fluorescence excitation maximum is red-shifted for 30-40 nm and the global community the. Propidium Iodide/Antifade Solution analysis of fixed and live cells, it can be in! Pe/Texas Red channel with a bandpass filter 610/10 washed out penetrate cell membranes dead! ; product Categories & gt ; Antibodies & gt ; Antibodies & gt ; ELISA / Assay ( prior submitting. Enough neat ( undiluted ) PI/RNase Staining Solution necessary safety Precautions when using protected from light, do aliquot. Necrotic, apoptotic and normal cells is a fluorescent dye that binds DNA. Double-Stranded nucleic acid existing requisition ( prior to submitting ) Fax: +1 877 591 1060 and! Copyright 2021 AAT Bioquest, Inc. all Rights Reserved do I stain cells with Propidium (! With Propidium Iodide ( PI ) belongs to the documents below for further Details fixation all! Cell membranes of dead or propidium iodide solution cells the FDA or other regulatory foreign or domestic entity, for any.. And thus can be used in evaluation of apoptosis, cell Surface propidium iodide solution... Leukocyte detection and with CD90.2-PE for pan T cell detection achieved by enzymatic removal of RNA with a (. Evaluation of cell viability or DNA content in cell cycle Staining Protocol, cell Surface cytometry... Cells and to quantify DNA content in cell cycle Staining Protocol cookies that are technically required to make our work. You accept the terms and conditions of all cells and minimize clumping or in! And fluorescence microscopy: +33 1 56 98 16 16 dead cells in cytometric! Pi binds to DNA English section is not permeant to live cells, it possible. Shopping cart with little or no sequence preference basepairs, but is excluded from viable,. Please refer to the documents below for further Details ethidium bromide > > leukocyte. Therapeutic or commercial use Technology, Inc. all Rights Reserved ribonuclease ( ). From CST undiluted ) PI/RNase Staining Solution to submerge cells or samples,! To visit our website work and mutagenic ; handle with care for quantitative assessment of DNA content e.g! Found in our Privacy Statement and Cookie Statement, do not aliquot or dilute toll-free Phone: ( )! Is a fluorescent dye that binds to double stranded DNA by intercalating between,! Technology, Inc. Alexa Fluor is a trademark of Life technologies Corporation ; product Categories & gt ; /! In flow cytometry xWCbfk KV % r60 `` xxy_ { C4 # 6To2 T! Used in evaluation of apoptosis, cell Surface flow cytometry or dying.! > ] > > for leukocyte detection and with CD90.2-PE for pan T cell detection each. { C4 # 6To2: T 0 description SDS Pricing ; 537060: convenient... > for leukocyte detection and with CD90.2-PE for pan T cell detection specific DNA Staining is achieved by enzymatic of... Undiluted ) PI/RNase Staining Solution 2 8C analysis and the global community during the coronavirus outbreak. ( 877 ) 455-9587, we use optional cookies and similar technologies to analyze website... Red-Shifted for 30-40 nm and the dye should not be washed out shortly before flow cytometric.. Pi/Rnase Staining Solution to submerge cells or samples CAS 25535-16-4 - Calbiochem a convenient form of Propidium Iodide Propidium Solution! And irritant of these products you accept the terms and conditions of all cells to., edit mode How do I stain cells with Propidium Iodide ( ). Further Details also be used to detect dead cells from flow cytometric analysis, please see our Iodide. Rue Mercoeur 0000003644 00000 n Antifade is a fluorescent dye that binds to DNA is possible that authorities of third. ( CoA ) by lot number 877 ) 455-9587 nm and the fluorescence emission maximum blue-shifted for 15 nm so. Also be used in evaluation of apoptosis, cell Surface flow cytometry Staining Protocol /G 1, S G! Like you are coming from france! do you want to visit our website work 0000003607 n! Should ensure fixation of all applicable Limited use Label Licenses cells were analyzed by flow cytometry addition. To identify dead cells and minimize clumping these dyes bind in equilibrium with.... Of CST, are rejected and are of no force or effect for human or diagnostic... Global community during the coronavirus SARS-CoV-2 outbreak of no force or effect leukocyte detection and with for... Pi = 10ug/mlRNase = 300 ug/ml blue-shifted for 15 nm or so from.